Customizable setup of advanced P-SUBmerged™ with Cronus Tower
For applications involving mammalian cell retention for expressing mAbs low RPM and preferably no bursting aeration bubbles is mandatory. The cell lines are fragile and in a perfusion setup benefit from.
• No HFF fouling – freedom to select broth velocities flushing out debris from inside the lumen
• No dead volumes – skip all the hoses and plastic connectors, SUBmerged and integrate the SepraPor HFF 100 % with Thalia diaphragm pump
• No local cell death - inverted Thalia pump above the HFF and SUB cannot accumulate cells
• Reduced shear force – remove all hoses and fittings and SUBmerge the HFF into the media inside the SUB
We introduce the SUBmerged - the HFF inlet are arranged SUBmerged in the broth inside the SUB and in the opposite end directly coupled to the diaphragm pump.
SUBmerged ATF + HFF + Cronus
The illustration above is the required units for the Cronus tower. Perfusion-Single-Use-Bioreactor available from sister company PerfuseCell™. The PC shown is running Linux-8 with Lucullus on top and thus not Windows.