CellMembra TFF with Clotho Tower
For applications involving mammalian cell retention for expressing mAbs - low RPM and preferably no bursting aeration bubbles is mandatory. The cell lines are fragile and in a perfusion setup benefit from.
- Eliminate HFF fouling– freedom to select broth velocities flushing out debris from inside the lumen regularly
- Reduced shear force– reduce the use of hoses and fittings
We introduce CellMembra with the customized SepraPor HFF integrated with the Clio SUP next to the SUB. The HFF being a part of the CLIO SUP reduce the hose connections with no coupling, hoses, etc.
The Clio diaphragm Single-Use-Pump is LASER guided and its unique performance is programmed selectively into the Clotho™ drive unit. The “CellMembra-300-DASbox” perfusion bioreactor operating in TFF mode available from sister company PerfuseCell™.
The above example is a 0.3 liter CellMembra designed specifically for the parallel DASbox. The OD76 mm BioBLU® is heavily customized and equipped with the SepraPor® HFF and the Clio™ SUP. DASbox takes care of agitation, aeration, pH, DO and media supply. The Clotho™ Tower controls Clio which takes care of TFF harvest and the HFF cleaning process and hereby protects the HFF. The pressurized air and vacuum required for Clotho is delivered by dual Alagonia and one Sarpedon. The Clotho unit operate as stand-alone and thus do not require interaction with the PCS.
Clotho Tower consist of these units - as needed:
- Clotho (or Lachesis) drive unit - drives both Thalia and Clio diaphragm pumps
- Alagonia pump which supply pressurized air - if needed
- Alagonia pump supplying vacuum
- Sarpedon reservoir