Control of two diaphragm Single-Use-Pump's

Dual channel SUP control station for Clio and Euterpe diaphragm Single-Use-Pump's. The hardware is named Metis and the software specific for the SUP to drive.

Software name

Clotho / Atropos 

Purpose of control

Twin SUPs like Clio and Euterpe

Drive gas pressure requirement

>1,2 - <4 bar atmospheric

Drive gas vacuum requirement

<0,1 bar absolute

Power supply

24 VDC, 2 amp

Hephaestus cabinet size

U2 (only 7,5 liter volume)

Weight, kilo



Clotho / Atropos-2 is a very compact control unit supporting the Clio and Euterpe type of Single-Use-Pump's. 

Clotho is the software in the Metis Drive Unit for Clio - integrated with the Perfusion-Single-Use-Bioreactor (P-SUB's) for high cell density cultivation. Such as the CellMembra P-SUB available by PerfuseCell. The P-SUB integrate the Clio diaphragm Single-Use-Pump (SUP), the Cross-Flow-Filter (CFF), the Single-Use-Sensor's (SUS) - all in one package ready to use. Clotho / Atropos control accurately the pumped volume and the velocity of the broth passing through the CFF.

Atropos is the software in the Metis Drive Unit for Euterpe - the unique stand-alone Positive Displacement Single-Use-Pump from company PumpCell. The Euterpe family is remarkable as there is no need for expensive external mass-flow sensor's. No need for tools exchanging the SUP cell. No need for bulky pump heads and sensor's. No nano size silicone particles polluting the liquid.

Driving Clio and Euterpe SUP's requires pressure above and vacuum below atmospheric pressure. The SUP drive gas pressure is continuously adjusted by two sets of proportional valves inside Clotho / Atropos. Intelligent microprocessor with advanced PID algorithms get information from Laser and pressure sensors.

Clotho / Atropos-2 stereo model in U2 Hephaestus Stainless Steel cabinet shown as rendered picture. In the middle shown as the real unit including the two red laser sensors. Further Clotho / Atropos-2 assembled with one Sarpedon reservoir and two Alagonia pumps in one compact tower. This fully independent setup ready to use right out of the box.


When Clotho / Atropos is connected to a pressure and vacuum source with sufficient capacity, then the spec is:

  • Stroke frequency, per minute: 1 - 60 (depending on pressured air / vacuum capacity)
  • Stroke duration, seconds: 1 - 60 (depending on pressured air / vacuum capacity)
  • Clio as integrated with CellMembra - CFF broth velocity, m/s: 0,1 – 15

Clio and Euterpe share the same diaphragm basic dimension. As to this also the same performance like SV ad CO.

Clio SUP size, name mini/30 small/100 medium/200 large/300
Euterpe SUP size, name  Euterpe-30 Euterpe-100 Euterpe-200  Euterpe-300
Stroke Volume, SV, mL/stroke 1-34 1-104 2-200  3-300
Cardiac Output, CO range, mL/min 1-750 2-1.500 3-3.000  4-4.000

Suitable drive gas pumps, as well in the Hephaestus cabinet, for supply of pressure and vacuum for Clotho / Atropos are Alagonia. Check out the Sarpedon reservoir stabilizing pressure/vacuum and reduce pulsation when several Clio or Euterpe SUPs operate on the same supply. Sufficient supply is your planned CO x 50% at >2 Bar pressure and CO x 50% at >900 mBar vacuum.

Clotho / Atropos can of course be driven by in lab installed pneumatic air and vacuum.


  • Internal 5” touch sensitive TFT display or external to PADs or smartPhones via Wi-Fi for programming and info from the built-in webserver
  • USB for Wi-Fi antenna and for software upgrade
  • RJ45 for IP/TCP via LAN and ModBus communication
  • Data collection / acquisition / control via OPC-UA via Cassiopeia to PC platform running Lucullus software from SecureCell

Communication – check out specific folder on this subject under Support
Price, availability and p/n – look under Commerce