Control of one diaphragm Single-Use-Pump

Single channel SUP control station for Clio or Thalia diaphragm Single-Use-Pump's (SUP).

Software name

Clotho or Lachesis

Purpose of control

Single SUP

Drive gas pressure requirement

  >1.2 - <4 bar atmospheric

Drive gas vacuum requirement

<0.1 bar absolute

Power supply

24 VDC, 2 amp

Hephaestus cabinet size

U2 (only 7.5 liter volume)

Weight, kilo



Clotho software control the Clio or Thalia LASER guide diaphragm Single-Use-Pump's integrated with the Perfusion-Single-Use-Bioreactor (P-SUB's) for high cell density cultivation.

  • The CellRetention P-SUB for ATF method integrate the Thalia diaphragm Single-Use-Pump (SUP)
  • The CellMembra P-SUB for TFF method integrate the Clio diaphragm Single-Use-Pump (SUP)

Both P-SUBs integrate further the Hollow-Fibre-Filter (HFF) and the pressure Single-Use-Sensors (SUS) - all in one package ready to use. Clotho controls accurately the pumped volume, number of strokes and the velocity. The Clio family is remarkable as there is no need for external mass-flow or pressure sensor's or even manual calibration is needed.

Driving diaphragm SUP's requires pressure above and vacuum below atmospheric pressure. The SUP drive gas pressure is continuously adjusted via a set of proportional valves inside Clotho. Intelligent microprocessor with advanced PID algorithms get information from Laser and internal pressure sensor's.

Left: Clotho-1 in U2 Hephaestus Stainless Steel cabinet as rendered picture. Middle: The Clotho Drive Unit as delivered with one laser sensor. Right; Typical setup with one Alagonia for vacuum or even two Alagonia pumps (vacuum and pressure) and one Sarpedon reservoir.


When Clotho is connected to a pressure and vacuum source with sufficient capacity, then the spec is:

  • Stroke frequency, per minute: 1 - 60 (depending on pressured air / vacuum capacity)
  • Stroke duration, seconds: 1 - 60 (depending on pressured air / vacuum capacity)
  • Clio as integrated with CellMembra - CFF broth velocity, m/s: 0,1 – 15

Clio as mounted on CellMembra and the stand-alone Clio O-SUP share the same diaphragm basic dimension. As to this also the same performance like SV ad CO.

SUP name Clio - Thalia Clio - Thalia Clio - Thalia Clio - Thalia
SUP size M80/60 M80 M100 M140
Stroke Volume, SV, mL/stroke   60 200 500
Cardiac Output, CO range, mL/min   1-750 5-1,500 10-4,000

Suitable Drive-Gas pumps, also in the Hephaestus cabinet, for supply of pressure and vacuum for Clotho are Alagonia. Check out the Sarpedon reservoir stabilizing pressure/vacuum and reduce pulsation when several Clio or Thalia SUPs operate on the same supply. Sufficient supply is your planned CO x 50% at >2 Bar pressure and CO x 50% at >950 mBar vacuum.

Clotho can of course be driven by in lab installed pneumatic air and vacuum.


  • Internal 5” touch sensitive TFT display or external to PADs or smart-Phones via Wi-Fi for programming and info from the built-in webserver
  • USB for Wi-Fi antenna and for software upgrades
  • RJ45 for IP/TCP via LAN and ModBus communication
  • Data collection / acquisition / control via OPC-UA via Cassiopeia to PC platform running software like Lucullus from SecureCell

Communication – check out specific folder on this subject under Support
Price, availability and p/n – look under Commerce